Effects of Culture Conditions on Growth and Docosahexaenoic Acid Production from Schizochytrium limacinum

The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and docosahexaenoic acid (C22: 6 n-3, DHA) production from Schizochytrium limacinum OUC88 were investigated in the present study. The results revealed that the optimal temperature, initial pH and salinity level of the medium for DHA production were 23 ℃, 7.0 and 18,respectively. Glucose was proved the best carbon source for the growth and DHA production from S. limacinum. Among the nitrogen sources tested, soybean cake hydrolysate, a cheap by-product, was found to be effective for the accumulation of DHA in S. limacinum cells. In addition, increasing the concentration of carbon sources in the medium caused a significant increase in cell biomass;however, accumulation of DHA in cells was mainly stimulated by the ratio of C/N in the medium. Under the optimal culture conditions, the maximum DHA yield achieved in flasks was 4.08 g L-1 after 5 d of cultivation.

作 者： ZHU Luying ZHANG Xuecheng REN Xueying ZHU Qinghua   作者單位： ZHU Luying(College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China;College of Life Sciences, Ludong University, Yantai 264025, P. R. China)

ZHANG Xuecheng,REN Xueying,ZHU Qinghua(College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China) 

刊 名： 中國(guó)海洋大學(xué)學(xué)報(bào)（英文版）  ISTIC 英文刊名： JOURNAL OF OCEAN UNIVERSITY OF CHINA  年，卷(期)： 2008 7(1)  分類(lèi)號(hào)： P7  關(guān)鍵詞： culture conditions   DHA production   Schizochytrium limacinum  

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